Leptospirosis is a bacterial disease, caused by various strains of the Leptospira species, a flexible, spiral-shaped, Gram-negative spirochete. L. pomona is the most common serovar affecting pigs; less commonly L.tarassovi infection may occur. The signs seen are as follows: abortion, stillbirths or the birth of live, weak piglets; kidney lesions (white spots) in grower/finisher pigs; rarely fever, jaundice and death may occur in young piglets.
VetPCR? LEPTOSPIRA Detection Kit is the direct detection of Leptospira spp. on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.
Characteristics
Ready to use : only DNA template and D.W. are
needed. Easy and speed protocol. Stable for 1
year at -20?C. Time-saving and cost-effective.
Contents
Interpretation of the Test Result
Expected PCR product size : 343 bp Fig. 1 Result:
Lane M: Brig? Molecular Weight Marker
Lane 1~2: LEPTOSPIRA Positive samples
Lane I.C.: Internal control
Lane P: Positive control
Lane N: Negative control
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