Flavobacterium columnare PCR Detection KitFlavobacterium columnare is a thin Gram-negative rod bacterium of the genus Flavobacterium. Fish with columnaris usually have brown to yellowish-brown lesions (sores) on their gills, skin and/or fins. The bacteria attach to the gill surface, grow in spreading patches, and eventually cover individual gill filaments. This results in cell death. A characteristic lesion produced by columnaris is a pale white band encircling the body, often referred to as saddleback condition. As the infection progresses, a yellowish-brown ulcer often is found in the center of the ?saddle?.
VetPCR? F. columnare Detection Kit is the direct detection of Flavobacterium columnare on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.
Characteristics Ready to use : only DNA template and D.W. are needed. Easy and speed protocol. Stable for 1 year at -20?C. Time-saving and cost-effective.
Contents
Interpretation of the Test Result Expected PCR product size : 252bp Fig. 1 Result: Lane M: Brig? Molecular Weight Marker Lane 1~2: F. columnare Positive samples Lane I.C.: Internal control Lane P: Positive control Lane N: Negative control
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