Nervous necrosis virus RT-PCR Detection KitViral nervous necrosis is a disease found world-wide in many species of marine fish, causing an extremely high mortality of affected larvae and juveniles. The agent of VNN has been identified as a new member of the Nodaviridae. Fish nodaviruses have been classified into four genotypes: tiger puffer nervous necrosis virus, striped jack nervous necrosis virus, barfin flounder nervous necrosis virus and red-spotted grouper nervous necrosis virus. Nerve tissue is the target tissue for nervous necrosis virus (NNV) infection, but NNV can also infect other tissues. The distribution of NNV in other tissues varies with fish species and age.
VetPCR? NNV Detection Kit is the direct detection of Nervous necrosis virus on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate, reliable technique.
Characteristics
Ready to use : only DNA template and D.W. are
needed. Easy and speed protocol. Stable for 1
year at -20?C. Time-saving and cost-effective.
Contents
Interpretation of the Test Result
Expected PCR product size : 238bp Fig. 1 Result:
Lane M: Brig? Molecular Weight Marker
Lane 1~2: NNV Positive samples
Lane I.C.: Internal control
Lane P: Positive control
Lane N: Negative control
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