Przeciwciała poliklonalne fosfo-specyficznye (custom phospho-specific antibody production) - PAKIET IV

Dostępność: Na zamówienie

Numer katalogowy: PL001-04

Stan magazynowy: 100 szt.

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Custom Phospho-specific Antibody Production

The Custom Phospho-specific Antibody Production package is designed for generating affinity-purified antibodies that specifically recognize the phosphorylated state of a protein. The package involves all of the services in Package I and II respectively, includes the synthesis, conjugation, and immunization of phospho peptides into rabbits.
Eight-week immunization protocol in a species of your choice, and an ELISA to verify titers on the 3^rd production bleed and post-affinity A titer of >1:50,000 is guaranteed if you use a peptide sequence we approved.

Detail	Total Time	Delivery
Synthesis of a Phospho Peptide Synthesis of a Non-Phospho Peptide	2 weeks	1-3mg lyophilized phospho-specific antibodies from two rabbits
Conjugation of the peptide to to KLH	1 week	5mg Pre-conjugated phospho-peptides and unmodified peptides
4 Immunizations per animal	12 weeks	2 ml pre-immune serum (lyophilized)
Production bleeds and affinity purification	1 week	MS and HPLC reports
Elisa Test		Elisa reports of 3^rd serum and post-affinity purification
Lyophilization and Shipping	1-2 weeks
Total Time	15-16 weeks

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