MAGnova Protein-G magnetic nano-particles
Novazym Polska
MAGnova Protein-G magnetic particles are an affinity matrix for the small-scale isolation and purification of immunoglobulins (IgG). A truncated form of recombinant Protein A is covalently coupled to a paramagnetic particle. Protein A exhibits high affinity for subclasses of IgG from many species including human, mouse, rat (1). The protein is coupled through a linkage that is stable and leak resistant over a wide pH range. This permits the immunomagnetic purification of IgGs from ascites, serum or cell culture supernatants; the matrix can be regenerated without loss of binding capacity. Protein A magnetic particles can be used to immunoprecipitate target proteins from crude cell lysates using selected primary antibody. In addition, specific antibodies can be chemically cross-linked to the Protein A coated surface to create a reusable immunoprecipitation bead, avoiding the co-elution of antibody with target antigen (2,3).