Anaplasma centrale is a Gram-negative bacterium of the Rickettsiales order lacking a traditional cell wall. Infection causes a mild form of anaplasmosis in cattle and has been used as a vaccine against A.marginale. It occurs primarily in warm tropical and subtropical areas. A. centrale is most commonly transmitted by ticks such as Boophilus spp. and Dermacentor spp. Cattle infection presents with fever, loss of appetite, jaundice, anemia and respiratory problems.
VetPCR? A.centrale Detection Kit is the direct detection of Anaplasma centrale on the basis of a genetic database, so it can diagnose very fast and accurately. It can amplify only specific gene using the PCR (Polymerase Chain Reaction) method, and take only 3 hours for detection. Therefore, it is a very fast, accurate and reliable technique.
Characteristics Ready to use : only DNA template and D.W. are
needed. Easy and speed protocol. Stable for 1
year at -20?C. Time-saving and cost-effective.
Contents
Interpretation of the Test Result Expected PCR product size : 275bp
Fig. 1 Result: Lane M: Brig? Molecular Weight Marker
Lane 1~2: A.centrale Positive samples
Lane I.C.: Internal control
Lane P: Positive control
Lane N: Negative control
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