Custom Phospho-specific Antibody Production
The Custom Phospho-specific Antibody Production package is designed for generating affinity-purified antibodies that specifically recognize the phosphorylated state of a protein. The package involves all of the services in Package I and II respectively, includes the synthesis, conjugation, and immunization of phospho peptides into rabbits.
Eight-week immunization protocol in a species of your choice, and an ELISA to verify titers on the 3rd production bleed and post-affinity A titer of >1:50,000 is guaranteed if you use a peptide sequence we approved.
Detail | Total Time | Delivery |
Synthesis of a Phospho Peptide Synthesis of a Non-Phospho Peptide |
2 weeks | 1-3mg lyophilized phospho-specific antibodies from two rabbits |
Conjugation of the peptide to to KLH |
1 week | 5mg Pre-conjugated phospho-peptides and unmodified peptides |
4 Immunizations per animal | 12 weeks | 2 ml pre-immune serum (lyophilized) |
Production bleeds and affinity purification | 1 week | MS and HPLC reports |
Elisa Test | Elisa reports of 3rd serum and post-affinity purification | |
Lyophilization and Shipping | 1-2 weeks | |
Total Time | 15-16 weeks |
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